1 Formaldehyde
The disinfection effect of formaldehyde gas is greatly affected by environmental temperature and humidity, and it is necessary to strictly control the temperature and humidity within the specified range during disinfection. When the temperature is above 20 ℃, formaldehyde gas can kill all microorganisms and their spores, but it does not inactivate protein. Formaldehyde disinfection takes a relatively long time and requires a relative humidity of around 70%.
Formaldehyde fumigation disinfection has become a common disinfection and sterilization method in China due to its advantages of broad-spectrum sterilization, convenient use, and low cost. However, due to the pungent odor of formaldehyde, its gas can irritate the eyes and mucous membranes, and has been confirmed to have a carcinogenic risk. After disinfection, it needs to be neutralized before ventilation. Due to residual formaldehyde after disinfection, attention should be paid to wiping and removing trace residues on the surface of objects.

2 Hydrogen peroxide
Hydrogen peroxide has the characteristics of broad-spectrum, high efficiency, quick acting, non-toxic, corrosive to metals and fabrics, greatly affected by organic matter, good stability of pure products, and unstable diluent. Adding other ingredients to existing products to stabilize hydrogen peroxide, enhance its bactericidal effect, and reduce its corrosiveness. The main advantages of hydrogen peroxide disinfection are dryness, rapid action, non toxicity, and no residue.
3 Chlorine dioxide
Chlorine dioxide is different from general chlorine containing disinfectants. It mainly exerts its disinfection effect through oxidation rather than chlorination, thereby avoiding the production of organic chlorides during the disinfection process. It has the characteristics of broad-spectrum, high efficiency, quick acting, corrosive to metals, bleaching effect on fabrics, and significant influence of organic matter on disinfection effect.

Sealed fumigation disinfection
Principle and Characteristics
The working principle of the sealed fumigation disinfection mode is to use the laboratory room as a unit, close the air supply and exhaust unit, air duct sealing valve, and laboratory door, so that the laboratory is in a sealed state. Disinfectant gas is generated inside the room or outside the room, and injected into the laboratory through a dedicated disinfection pipeline.
2. There are issues
Several high-level biosafety laboratory users in China have found confusion that when using closed fumigation disinfection, if there are multiple core workspaces and no multiple disinfection equipment available for simultaneous disinfection, there is a risk of contamination between the disinfected, disinfected, and unsterilized rooms, and even the surrounding environment such as ceilings and peripheral corridors.

How to solve it
The application of hydrogen peroxide sterilization technology in laboratory environment disinfection in Europe has a history of nearly 20 years, especially in recent years, with the rapid development of hydrogen peroxide sterilization technology in the context of the EU's restriction on the use of formaldehyde space sterilization.

Scope of application of hydrogen peroxide sterilization system

1. Suitable for high-level biological laboratories, cell rooms, microbiology rooms, sterile rooms, etc.
2. Can be used for disinfection of sterile laboratory environments, microbiology rooms, sterile rooms, isolators, etc RABS、 Disinfection and sterilization of sterile transfer chambers.
3. It can be safely used for disinfecting various surfaces and spaces such as stainless steel, plastic, glass, floors, walls, etc.
Compared with the traditional disinfectant formaldehyde, hydrogen peroxide has the advantages of high disinfection efficiency, quick effect, environmental protection, and convenient use. The number of users using this disinfectant for high-level biosafety laboratory disinfection is gradually increasing.